Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 4. lncRNA ZFAS1 interacts with and negatively regulates miR-150-5p. (A) Sequence alignment of lncRNA ZFAS1 with the binding sites in miR-150-5p; (B) The expression of miR-150-5p in lung tissues was analyzed by RT-qPCR; (C) Spearman correlation was used to analyze the association between miR-150-5p and lncRNA ZFAS1 expression in the lung tissues of BLM-induced pulmonary fibrosis; (D) RT-qPCR was used to detect the expression of miR-150-5p in HFL1 cells; (E) FISH was performed to evaluate the location of endogenous lncRNA ZFAS1 and miR-150-5p in HFL1 cells; (F) RT-qPCR was applied to detect the expression of lncRNA ZFAS1 and miR-150-5p in AGO2 pellet; (G) dual-luciferase reporter gene was used to verify the targeted relationship between lncRNA ZFAS1 and miR-150-5p; (H) RNA pulldown assay showed that biotin labeled miR-150-5p-WT interacted with lncRNA ZFAS1. ***P<0.001, compared with the control group; ##P<0.01, compared with NC group; ΔΔΔP<0.001, compared with IgG group; ★★★P<0.001, compared with bio-NC group.