Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 5. SLC38A1 is a target gene of miR-150-5p. (A) Bioinformatics analysis indicated the putative binding sites and corresponding mutant region for miR-150-5p within SLC38A1; (B) dual-luciferase reporter gene was used to verify the targeted relationship between miR-150-5p and SLC38A1; (C, D) The effect of miR-150-5p on the expression of SLC38A1 protein was determined by western blot; (E) Spearman analysis was used to analyze the association between miR-150-5p and SLC38A1 expression in the lung tissues of BLM-induced pulmonary fibrosis. **P<0.01, compared with the NC group.