Research Paper Volume 12, Issue 10 pp 9103—9124

A novel 3’,5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer

Figure 1. Effects of compound 10 on PCa cell viability and proliferation. (A) PC3, DU145 and RWPE-1 cells were treated with various concentrations of C10 for 24, 48 or 72 h, and cell viability was analyzed with an MTT assay. (B) Chemical structure of flavagline-like compound 10. (C) The IC50 values (μM) of the indicated cell lines were measured at three time points (24, 48 and 72 h) and summarized in a table. (D, E) C10 significantly inhibited the proliferation of PC3 and DU145 cells. A clonogenic assay was performed, and the number of colonies formed was analyzed. Per condition, three independent experiments were performed. Data are shown as the mean ± SD, *P < 0.05, **P < 0.01 vs. the control group.