Figure 3. C10 induced sub-G1 phase arrest of PC3 cells in culture. (A, B) PC3 cells were treated with C10 (0, 4, 6, 8, 10 or 12 μM) for 24 h and stained with PI so that the DNA content could be analyzed by flow cytometry. C10 increased the proportion of PC3 cells in sub-G1 phase of the cell cycle. (C–F) Western blots of PC3 cells treated for 24 h with C10. The blots were probed with antibodies against CDK2, CDK6, c-Myc, Cyclin D1, Cyclin D2, PCNA, P21cip1 and P27kip1. All data shown are representative of three independent experiments. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01 vs. the control group.