Research Paper Volume 12, Issue 10 pp 9103—9124

A novel 3’,5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer

Figure 4. C10 induced apoptosis in PC3 cells. (A, B) PC3 cells were treated with C10 (0, 4, 6, 8, 10 or 12 μM) for 24 h, stained with annexin-V-FITC and PI, and then analyzed by flow cytometry. C10 dose-dependently increased the percentage of annexin-V-FITC-positive apoptotic cells. (C, D) Western blot showing the expression of PARP, cleaved PARP, Caspase-3, cleaved Caspase-3, Caspase-8, cleaved Caspase-8, Caspase-9, cleaved Caspase-9, Bcl-2, Bax, cytochrome C and Survivin in PC3 cells treated with C10 for 24 h. (E, F) The phosphorylation levels of core factors in the MAPK signaling pathway (P38/MAPK and ERK1/2) were detected at different time points. β-actin was used as a loading control. Relative expression was determined based on the band intensity compared with that of the loading control. All data shown are representative of three independent experiments. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01 vs. the control group.