Research Paper Volume 12, Issue 10 pp 9103—9124

A novel 3’,5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer

Figure 6. PKCδ induced PCD by activating JNK signaling in C10-treated PC3 cells. (A, B) Western blot of PC3 cells treated for the indicated times (0, 2, 4, 8, 16 or 24 h) with C10. IL-6, p-SAPK/JNK and SAPK/JNK antibodies were used. (C) Cultured PC3 cells were treated with C10 in the presence of different inhibitors (siPKCδ and the JNK-specific inhibitor Tanzisertib [CC-930]) for 24 h. The cells were then stained with Hoechst 33258 and photographed using a fluorescence microscope (magnification ×200, scale bar: 100 μm). (D, E) Cultured PC3 cells were stained with annexin-V-FITC and PI for flow cytometry analysis. (F) The mRNA levels of PKCδ, Caspase-9, IL-6, IL-8, IL-1β and Bax were measured by qRT-PCR. All data shown are representative of three independent experiments. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01 vs. the control group.