Research Paper Volume 12, Issue 10 pp 9103—9124

A novel 3’,5’-diprenylated chalcone induces concurrent apoptosis and GSDME-dependent pyroptosis through activating PKCδ/JNK signal in prostate cancer

Figure 7. Inhibition of PKCδ suppressed C10-induced concurrent apoptosis and GSDME-dependent pyroptosis in PC3 cells. (A, B) Cultured PC3 cells were incubated with C10 in the presence of siPKCδ or the JNK-specific inhibitor Tanzisertib (CC-930) for 24 h to determine the link between the apoptotic and pyroptotic pathways. The protein levels of PKCδ, p-SAPK/JNK, SAPK/JNK, Survivin, Bax, PARP, cleaved PARP, Caspase-3, cleaved Caspase-3, IL-6, GSDME and GSDME-N were examined by Western blotting. (C) LDH enzyme activity was measured in the culture supernatants of PC3 cells after various treatments. (D, E) The phosphorylation levels of core factors in the MAPK signaling pathway (P38/MAPK and ERK1/2) were detected after various treatments in PC3 cells. β-actin was used as a loading control. All data shown are representative of three independent experiments. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01 vs. the control group.