Figure 4. Effects of LEO on acetylcholinesterase content in APP/PS1 mice and WT mice. Hippocampus of each group was extracted by extracting buffer and then estimated by Western blotting. (A) Representative Western blotting of acetylcholinesterase. (B) Densitometric analyses of the immunoreactivity to the antibody shown in A. (C) Quantitative analysis for the relative intensity of AChE in hippocampus. (D) Immunostaining with anti-AChE antibody in each group. Scale bar, hippocampus = 100 μm, CA1, CA3 = 20 μm, DG = 50 μm respectively. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different from non-treated mice group. LEO: lemon essential oil.