Figure 5. Synaptic density is significantly impaired in APP/PS1 mice and repaired after LEO treatment. IHC analysis for the expression and localization of both presynaptic marker, synaptophysin and postsynaptic marker, PSD95 in hippocampus (A), hippocampal CA1 region (B), hippocampal CA3 region (C) and hippocampal DG region (D), cortex region (E). (F) Western blot confirmation for PSD95, synapsin-1 and synaptophysin expression in hippocampus from APP/PS1 mice and WT mice. (G–H) Quantitative analysis of Western blots analysis for hippocampus. (I, J) Quantitative analysis for the relative intensity of synaptophysin, PSD-95, and SYN/PSD95 contacts which represents synaptic density (K) compared with WT mice. Scale bar, A–D = 100 μm, B = 20 μm, C–E = 50 μm. Values are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. LEO: lemon essential oil.