Research Paper Volume 12, Issue 9 pp 8622—8639

Lemon essential oil ameliorates age-associated cognitive dysfunction via modulating hippocampal synaptic density and inhibiting acetylcholinesterase

Figure 6. Neurotrophic signaling is significantly increased after LEO treatment in APP/PS1 mice and WT mice. (A) Hippocampus lysates were obtained after behavioral experiment and probed with antibodies detecting TrkB, BDNF, total GSK-3β, p-GSK-3β, total AKT, p-AKT, total ERK, p-ERK, total GSK-3β and p-GSK-3β levels in APP/PS1 and WT mice. Further quantitative analysis was conducted, with percentage changes versus WT mice shown in (BF). (G) Immunostaining analysis result of H. (H) Immunostaining with TrkB antibody in each group. Values are means ± SEM of determinations from each group. *P < 0.05, **P < 0.01, ***P < 0.001. LEO: lemon essential oil.