Figure 5. Overexpression of Klotho alleviates IS-induced heart failure and kidney damage in vivo. (A) Serum CR and BUN levels detected by ELISA. (B) Urine CR and BUN levels detected by ELISA. (C, D) Analysis of renal fibrosis by Masson staining. (E) Expression of α-SMA in kidney tissue evaluated by western blotting. (F) Expression of α-SMA relative to GAPDH in kidney tissue. (G) Serum BNP level detected by ELISA. (H) Heart weight was calculated in each treatment group. (I) Heart tissue was stained with hematoxylin and eosin. *P < 0.05, **P < 0.01.