Figure 6. Overexpression of Klotho alleviates IS-induced heart failure and kidney damage in vitro and in vivo by activating the NF-kB pathway. (A) THP-1 cells were exposed to PMA (160 nM) for 48 h, incubated in PMA-free medium for 24 h, and then transfected with the Klotho expression plasmid for 24 h. The expression of p-p65 in cells was evaluated by western blotting. (B) The relative expression of p-p65 was evaluated normalized to p65. (C) Quantification of relative expression based on F4/80, p65 and DAPI staining in mouse kidney tissue. **P < 0.01.