Figure 4. High-fat diet and RS2 supplemented with high-fat diet altered the microbial community at the family and genus levels in aged mice. (A) Relative abundance of gut microbiota in family level (those with abundance >1% are presented) among the three groups. (B) Top 35 taxa with the highest abundance at the genus level among the three groups. Colors were expressed by lg calculation. (C) Generic taxa with higher mean proportions in the HF and lower mean proportions in the HFRS groups compared to the CON group by further posttest comparisons. (D) Generic taxa with lower mean proportions in the HFRS compared to the HF and the CON groups by further posttest comparisons. (E) Generic taxa with lower mean proportions in the HF and the HFRS compared to the CON group by further posttests comparisons. N=4 to 6 per group. Differences were compared by Kruskal–Wallis H test with Dunn’s multiple posttest comparisons between the two groups. * p<0.05, ** p<0.01. CON, control group; HF, high-fat diet group; HFRS, high-fat diet+20%RS2 group.