Figure 5. High-fat diet and RS2 supplemented with high-fat diet altered the microbial metabolism in aged mice. (A) Functional prediction analyses based on the occurrence of clusters of orthologous groups (COGs) of proteins in microbiota among the three groups (those with abundance >1% are presented). (B) Colon short-chain fatty acid levels regulated by HF and HFRS diets. (C) Cecal short chain fatty acid levels regulated by HF and HFRS diets. N=4 to 6 per group. Data are expressed as mean + SE. Differences were compared by one-way ANOVA among the three groups with Tukey’s multiple comparison posttests between two groups or Kruskal–Wallis H test with Dunn’s multiple comparisons posttests between two groups. * p<0.05, ** p<0.01 compared with HFRS or CON. CON, control group; HF, high-fat diet group; HFRS, high-fat diet+20%RS2 group; SCFA, short-chain fatty acid.