Figure 1. Col10a1-TAp63γ expression plasmid and establishment of stable TAp63γ expressing ATDC5 cell lines. (A) pCMV-TAp63γ and its derivative pCol10a1-TAp63γ expression plasmids are shown. Enzyme restriction sites for cloning are also shown. (B) Enzyme digestion confirmed the integration of TAp63γ in designated stable cell lines. (C) PCR using p63 and Taq sequence-specific primers confirmed the integration of TAp63γ into the stable cell lines: pCMV-TAp63γ and Col10a1-TAp63γ. (D) Western blot results further confirmed expression of TAp63γ in designated stable lines.