Research Paper Volume 12, Issue 9 pp 8669—8679

TAp63γ influences mouse cartilage development

Figure 5. In vitro effect of TAp63γ on matrix mineralization. (A) ATD5C cells were cultured for 0, 4, 7, 14, or 21 days and stained with Alizarin red. After 4 and 7 days in culture, enhanced Alizarin red staining was observed in both Col10a1-TAp63γ and pCMV-TAp63γ stable cell lines compared with the blank and vector controls. Scale bar, 25 μm. (B) Sum object area of the staining by densitometry analysis (n=3, * p<0.05, ** p<0.01).