Figure 3. KTN1-AS1 affected cell proliferation and apoptosis. (A) qRT-PCR analysis of the expression of KTN1-AS1 in A549 and H1299 cell lines after si-KTN1-AS1. (B) The proliferation vitality detected using the CCK-8 assay. (C) EdU assays fro the determination of the cell proliferation ability of si-KTN1-AS1 transfected cells. (D) Colony formation assays for the functional exploration of knockdown of KTN1-AS1 on cellular growth. (E) The cells apoptosis was analyzed by flow cytometry. (F) The activities of caspase 3/9 were determined. * P < 0.05, **P < 0.01.