Research Paper Volume 12, Issue 9 pp 8680—8701

STAT1-induced upregulation of lncRNA KTN1-AS1 predicts poor prognosis and facilitates non-small cell lung cancer progression via miR-23b/DEPDC1 axis

Figure 7. MiR-23b directly targeted DEPDC1 in NSCLC cells. (A) Heatmap and volcano map of DEGs using TCGA data analysis. (B) Veen diagram. (C) Heatmap and volcano map of DEGs using GSE18842 and GSE33532 data analysis. (D) Intersection of 73 commonly expressed genes and up-regulated genes in GSE18842 and GSE33532. (E) Overall survivals were analyzed by GEPIA. (F) starBase program analyzed the expressing correlation between miR-23b and 6 genes. (G) Relative levels of DEPDC1 in TCGA data, GSE18842 and GSE33532. (H) qPCR evaluated the DEPDC1 levels in 127 NSCLC tissues. (I) The three binding sites between miR-23b and 3’UTR of DEPDC1 mRNA were predicted by starBase program. (J) Luciferase activity detection. (K) qPCR evaluated the DEPDC1 levels in A549 and H1299 cells after transfection with miR-23b mimics and inhibitors. * P < 0.05, **P < 0.01.