Figure 6. TIMP1 expression was associated with mesenchymal phenotype in ccRCC cell lines. Relative mRNA expression of TIMP1 (A), E-cadherin (B) and N-cadherin (C) in 2 ccRCC cell lines and a normal cell line presented separately as histograms. (D) TIMP1, E-cadherin, and N-cadherin protein levels as determined by Western blot analysis. β-actin was used as the internal control. (* p<0.05, ** p<0.01, *** p<0.001 by t-test).