Figure 4. MEX3A knockdown may inhibit ESCC through targeting CDK6. (A) Heatmap of the RNA sequencing performed on Eca-109 cells with or without MEX3A knockdown (3 v 3). (B) The mRNA expression of several most significantly down-regulated DEGs in shMEX3A group identified by RNA sequencing was further detected in TE-1 cells. (C) The protein levels of various down-regulated DEGs in shMEX3A groups were verified in TE-1 cells through western blotting. (D) The MEX3A-related interaction network was predicted based on IPA analysis. (E) The expression of CDK6 in ESCC tumor tissues and normal tissues was detected and compared by IHC analysis. (F) The background expression of CDK6 in ESCC cell lines including Eca-109, EC-9706, KYSE-450 and TE-1 was detected by qPCR. (G) Co-IP assay was performed to verify the direct interaction between MEX3A and CDK6. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.