Research Paper Volume 12, Issue 21 pp 21091—21113

MEX3A is upregulated in esophageal squamous cell carcinoma (ESCC) and promotes development and progression of ESCC through targeting CDK6

Figure 5. Knockdown of CDK6 inhibited development of ESCC in vitro. (A) The efficiency of CDK6 knockdown in Eca-109 cells was detected by qPCR. (B) The knockdown of CDK6 in Eca-109 cells was confirmed by western blotting. (C) The inhibition of cell proliferation by CDK6 knockdown in Eca-109 cells was evaluated by Celigo cell counting assay. (D) The influence of colony formation ability of Eca-109 cells by CDK6 knockdown was examined by colony formation assay. (E, F) The effects of CDK6 knockdown on cell migration of Eca-109 cells were assessed by wound-healing (E) and Transwell (F) assays. (G) Flow cytometry was performed to detect cell apoptosis of Eca-109 cells with or without CDK6 knockdown. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.