Figure 5. Knockdown of CDK6 inhibited development of ESCC in vitro. (A) The efficiency of CDK6 knockdown in Eca-109 cells was detected by qPCR. (B) The knockdown of CDK6 in Eca-109 cells was confirmed by western blotting. (C) The inhibition of cell proliferation by CDK6 knockdown in Eca-109 cells was evaluated by Celigo cell counting assay. (D) The influence of colony formation ability of Eca-109 cells by CDK6 knockdown was examined by colony formation assay. (E, F) The effects of CDK6 knockdown on cell migration of Eca-109 cells were assessed by wound-healing (E) and Transwell (F) assays. (G) Flow cytometry was performed to detect cell apoptosis of Eca-109 cells with or without CDK6 knockdown. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.