Research Paper Volume 12, Issue 9 pp 8710—8727

Soy isoflavones improve the oxidative stress induced hypothalamic inflammation and apoptosis in high fat diet-induced obese male mice through PGC1-alpha pathway

Figure 4. Soy isoflavones could reduce apoptosis in hypothalamus of DIO male mice. (A) Quantification shows the mRNA levels of apoptosis factors in hypothalamus of DIO mice fed with basal diets and the addition with different doses of soy isoflavones; (B, C) Western blots and quantification show the apoptosis factors protein levels in hypothalamus of DIO mice fed with basal diets and the addition with different doses of soy isoflavones; (D, E) Western blots and quantification show the phosphorylation levels of AKT in hypothalamus of DIO mice fed with basal diets and the addition with different doses of soy isoflavones; (F) Quantification shows the mRNA levels of FAS and FASL in hypothalamus of DIO mice fed with basal diets and the addition with different doses of soy isoflavones; (G, H) Western blots and quantification show the protein levels of FAS and FASL in hypothalamus of DIO mice fed with basal diets and the addition with different doses of soy isoflavones; * p < 0.05, ** p < 0.01, ***p < 0.001.