Research Paper Volume 12, Issue 9 pp 8710—8727

Soy isoflavones improve the oxidative stress induced hypothalamic inflammation and apoptosis in high fat diet-induced obese male mice through PGC1-alpha pathway

Figure 5. Soy isoflavones could improve palmitic acid-induced apoptosis in hypothalamic cell line. (A) Quantification shows the cell viability of N42 cells treated with PA(100μM), respectively, alone or in combination with daidzein(50μM) or genistein(50μM) for 24h. (B, C) Representative images and quantification show the ROS production of N42 cells under treatment with PA(100μM), respectively, alone or in combination with daidzein(50μM) or genistein(50μM) for 24h by dihydroethidium labeling. Scale bar: 50μm. (D, E) Representative images and quantifications show the nuclear morphology of N42 cells under treatment with PA(100μM), respectively, alone or in combination with daidzein(50μM) or genistein(50μM) for 24h. * p < 0.05, ** p < 0.01, ***p < 0.001. Scale bar: 50μm.