Figure 3. Downregulated expression of PRMT5 inhibited the proliferation and aggressiveness of bladder cancer cells. (A) PRMT5 was efficiently knocked down in T24-siRNA and Biu87-siRNA cells. (B–D) Cell proliferation was determined by EdU assay, CCK-8 assay, and colony formation assay of bladder cancer cells. (E, F) Cell invasion and migration capacities were determined using the transwell invasion assay and wound-healing assay for the indicated bladder cancer cells. Error bars show the standard error of the mean. siC: PRMT5-siRNA/negative control; si-1: PRMT5-siRNA/#1; si-2: PRMT5-siRNA/#2. *P < 0.05, **P < 0.01.