Research Paper Volume 12, Issue 9 pp 8728—8741

High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer

Figure 3. Downregulated expression of PRMT5 inhibited the proliferation and aggressiveness of bladder cancer cells. (A) PRMT5 was efficiently knocked down in T24-siRNA and Biu87-siRNA cells. (B–D) Cell proliferation was determined by EdU assay, CCK-8 assay, and colony formation assay of bladder cancer cells. (E, F) Cell invasion and migration capacities were determined using the transwell invasion assay and wound-healing assay for the indicated bladder cancer cells. Error bars show the standard error of the mean. siC: PRMT5-siRNA/negative control; si-1: PRMT5-siRNA/#1; si-2: PRMT5-siRNA/#2. *P < 0.05, **P < 0.01.