Research Paper Volume 12, Issue 9 pp 8728—8741

High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer

Figure 6. High PRMT5 expression in bladder cancer promoted tumor progression through the cell cycle and the mTOR signaling pathway. (A, B) GSEA indicated that high or low PRMT5 expression in bladder cancer is correlated with the cell cycle and the mTOR signaling pathway. Values were row-scaled to show the relative expression. (C) Flow cytometric analysis of the cell cycle showed that PRMT5 knockdown caused G1 cell cycle arrest. (D) PRMT5 facilitated the G1 to S phase transition of the cell cycle in bladder cancer. Corresponding statistics of knockdown (E) and overexpression (F) are presented by a bar graph (*P < 0.05, **P < 0.01, ***P < 0.001). (G, H) Western blots were performed after knockdown or overexpression of PRMT5 to evaluate the relevant protein expression of the cell cycle and the mTOR signaling pathway.