Research Paper Volume 12, Issue 10 pp 9275—9291

NAC1 attenuates BCL6 negative autoregulation and functions as a BCL6 coactivator of FOXQ1 transcription in cancer cells

Figure 1. FOXQ1 promoter has BCL6 binding motifs. (A) The BCL6 consensus motif was retrieved with Cistrome analysis and the position frequency matrix of BCL6 motif is presented in the left panel. On the right panel the consensus sequence of BCL6 binding motifs on FOXQ1 promoter resulting from the Cistrome analysis is represented. Below, a schematic map was provided to show Positions A/B/C of FOXQ1 promoter loci. (B) Chromatin immunoprecipitation with anti-BCL6 and IgG antibodies in MCF7 and HeLa cells. (C) Semi-quantitative PCR was used to evaluate the enrichment of the three different putative BCL6 binding motifs of the FOXQ1 promoter.