Figure 2. Co-immunoprecipitation (co-IP) analysis of interactions of NAC1 with BCL6. (A) Representation of the NAC1 domain mapping corresponding to the different NAC1 partial constructs used. (B) HEK293T cells were transfected with NAC1-V5 and BCL6-Flag constructs, and the lysates were collected for reciprocal protein co-IP experiments. (C) Reciprocal co-IP of full-length NAC1 and NAC1- deletion mutants with BCL6.