Figure 1. Scatterplots of CSF Ng levels by clinical diagnosis and biological status. (A) Mean CSF Ng levels were higher in AD subjects compared with sMCI subjects (P = 0.011) or CN controls (P < 0.001). Mean CSF Ng levels were higher in pMCI subjects compared with sMCI subjects (P = 0.028) or CN controls (P < 0.001). Mean CSF Ng levels were higher in sMCI subjects compared with CN controls (P = 0.042). (B) When comparing by Aβ status, Ng values were differentially increased in Aβ+ CN (P = 0.032) and Aβ+ MCI individuals (P < 0.001), whereas in the dementia stage, Ng levels were elevated regardless of Aβ status (P = 0.243). (C) Mean CSF Ng levels were higher in those with A+T+ (Mean [SD]: 608.7 [345.0] pg/mL, n = 230) compared with those with A-T- (Mean [SD]: 260.5 [175.6] pg/mL; n = 99) (P < 0.001). Mann-Whitney U test/Kruskal-Wallis test was used for all group comparisons. * p<0.05, *** p<0.001. Abbreviations: CN, cognitively normal; MCI, mild cognitive impairment; sMCI, stable MCI; pMCI, progressive MCI, MCI progressing to dementia due to AD; AD, Alzheimer’s disease; CSF: cerebrospinal fluid; Ng, neurogranin; Aβ, amyloid-β; A-, amyloid-β negative (CSF Aβ>192 pg/mL); A+, amyloid-β positive (CSF Aβ<192 pg/mL); T-, tau negative (CSF p-tau<23 pg/mL); T+, tau positive (CSF p-tau>23 pg/mL).