Figure 1. Vastus lateralis muscle tissue NAD+ concentrations (A), NADH concentrations (B), and NAMPT protein levels (C) in middle-aged (MA) participants prior to (Pre) and following 10 weeks of resistance training (Post) as well as basal values in college-aged participants that were recreationally trained. All data are presented as means±SD values. Panel (D) shows a representative Western blot for n=3 middle-aged participants (Pre and Post training) as well as n=3 college-aged participants. All middle-aged participants were assayed for NAMPT; however, 15/16 middle-aged and 14/15 college-aged participants were assayed for muscle NAD+ and NADH concentrations due to tissue limitations. Symbols: #, indicates value in older group at Pre and/or Post training was less than college-aged cohort (p<0.05).