Research Paper Volume 12, Issue 10 pp 9447—9460

Resistance training increases muscle NAD+ and NADH concentrations as well as NAMPT protein levels and global sirtuin activity in middle-aged, overweight, untrained individuals

Figure 2. Vastus lateralis muscle tissue SIRT1 protein levels (A), SIRT3 protein levels (B), global sirtuin (SIRT) activity (C), and acetylated protein levels (D) in middle-aged (MA) participants prior to (Pre) and following 10 weeks of resistance training (Post) as well as basal values in college-aged participants that were recreationally trained. All data are presented as means±SD values. Panel (E) shows representative Western blots for n=2 middle-aged participants (pre and Post) as well as n=2 college-aged participants. All middle-aged participants were assayed; however, 14/15 college-aged participants were assayed due to tissue limitations.