Research Paper Volume 12, Issue 10 pp 9475—9488

Amphiregulin promotes cisplatin chemoresistance by upregulating ABCB1 expression in human chondrosarcoma

Figure 2. Knockdown of amphiregulin expression suppresses cisplatin resistance in human chondrosarcoma cells. (A) Intracellular AR levels in whole cell lysates were analyzed by Western blot and qPCR assays. (B) Chondrosarcoma cells were treated with different concentrations of cisplatin for 24 h and cell viability was analyzed using the MTT assay. (C) Cell proliferation rates were determined by the MTT assay. (DF) Chondrosarcoma cells were treated with cisplatin (10 μM) for 24 h and cell apoptosis was examined by caspase-3 activity (D), PI staining (E), and Annexin V-FITC binding. (F) The results were obtained from 3 independent experiments and are expressed as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with controls.