Research Paper Volume 12, Issue 10 pp 9515—9533

Figure 5. CQ attenuated MPTP toxicity by decreasing ROS level in vitro. (A, E) Cell viability was determined for SK-N-SH cells treated with different concentration of H₂O₂ (0, 10, 20, 50, 100, 200, 500μM) or MPP⁺ (0, 0.1, 0.2, 0.5, 1, 2, 5mM) for 24h, respectively. (B, F) Low dose of CQ (5μM) increased cell viability in the absence or presence of H₂O₂ (200μM) or MPP⁺(2mM) tested by CCK-8 kit, respectively. (C, G) CQ decreased cell death tested by Hoechst staining after the exposure of H₂O₂ (200μM) or MPP⁺(2mM) for 24h, respectively. (D, H) Representative images of Hoechst staining for SK-N-SH cells treated with H₂O₂ (200μM) or MPP⁺(2mM), respectively. (I–K) CQ decreased ROS in the absence or presence of MPP⁺(2mM) tested by flow cytometry, microplate reader, and microscope, respectively. Data expressed as the mean ± SD. *P<0.05, **P<0.01, indicate significant difference. Scale bar=50μm.