Research Paper Volume 12, Issue 10 pp 9633—9657

MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer

Figure 3. MiR-214-3p suppresses CRC cell metastasis in vitro and in vivo. (AC) Transwell assays revealed that miR-214-3p suppressed CRC cell migration and invasion abilities. (DE) Western blot analysis revealed that miR-214-3p decreased the expression of N-cadherin and vimentin and increased the expression of E-cadherin and Zo1. (FI) More lung metastatic nodules were observed in the LV-miR-214-3p group than in the LV-INC group. (JK) IHC analysis indicated that the expression of E-cadherin was lower and that the expression of N-cadherin was higher in xenograft subcutaneous tissues from the LV-miR-214-3p inhibitor group. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.