Research Paper Volume 12, Issue 10 pp 9633—9657
MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer
Figure 4. MiR-214-3p directly targets PLAGL2. (A) Based on the public bioinformatics algorithms, seven potential targets of miR-214-3p were selected. (B–F) The Starbase 3.0 database and our study indicated that the expression levels of PLAGL2 and MYH9 were higher in cancer tissues than in normal tissues. (G–I) The mRNA and protein levels of PLAGL2 were inhibited by miR-214-3p. (J) IHC analysis indicated that the expression of PLAGL2 could be inhibited by miR-214-3p in xenograft subcutaneous tissues. (K) qRT-PCR analysis demonstrated that PLAGL2 was negatively correlated with miR-214-3p expression in CRC tissues. (L–M) Dual luciferase reporter assays in CRC cells. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.