Figure 7. MYH9 is a direct target gene regulated by PLAGL2. (A–B) WB and qRT-PCR analyses indicated that the expression of MYH9 was regulated by miR-214-3p and PLAGL2. (C–D) The inhibitory effect of miR-214-3p on MYH9 could be reversed by Sh-PLAGL2. (E–F) qRT-PCR analysis demonstrated that MYH9 was correlated with the expression of miR-214-3p and PLAGL2 in CRC tissues. (G–H) ChIP assays with PLAGL2 antibody or IgG were performed to verify the binding between PLAGL2 and the MYH9 promoter in SW480 cells. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.