Figure 12. The in vitro wound healing ability of the OSE is compromised with age. (A) Ovary pieces encapsulated in alginate hydrogel beads. (B) Representative images of Troma-1 IHC with reproductively young (n = 7) and old ovary pieces (n = 7) at Day 0 and Day 8 of culture. Ovaries were fixed at each timepoint, therefore, the ovaries are shown at D0 and D8 are not the same. Scale bars are 100 μm. (C) Graph showing the average percent area of ovarian pieces encapsulated by Troma-1 positive cells. T-tests were performed; asterisk denotes significance (P = 0.029). Data are represented as mean ± SEM.