Research Paper Volume 12, Issue 10 pp 9686—9713

Ovulation and ovarian wound healing are impaired with advanced reproductive age

Figure 14. COX2 expression is decreased in reproductively old ovaries. (A) Representative image of a reproductively young ovary stained for COX2 expression. (B) Magnified image of boxed region in (A), a site of recent follicle rupture. (C) Representative image of a reproductively old ovary stained for COX2 expression. (D) Magnified image of boxed region in (C). The insets are IgG controls of the boxed regions. Scale bars are (A, C) 100 μm and (B, D) 25 μm. (E) Graph showing the reciprocal intensity values of recently ruptured follicles and formed CLs in tissue from reproductively young and old mice. A.u. stands for arbitrary units. A t-test was performed; asterisks indicate P < 0.0001. Data are represented as mean ± SEM.