Figure 3. Ovaries from reproductively old mice contain fewer CLs than young mice. (A) Representative H&E-stained ovarian tissue sections demonstrate the CL tracking process. Numbers in the upper left corner indicate the tissue section number. Black asterisks identify the same CL that appears across tissue sections. Indicated CL was visible beginning in section 75 and ending in section 240. Scale bars are 100 μm. (B) Graph showing the number of CLs per ovary for both age cohorts. A t-test was performed; asterisks denote significance (P = 0.008) (N = 5 mice, 10 ovaries per age group). Data are represented as mean ± SEM. The number of CLs per ovary for all (C) reproductively young and (D) reproductively old mice.