Research Paper Volume 12, Issue 10 pp 9686—9713

Ovulation and ovarian wound healing are impaired with advanced reproductive age

Figure 5. Reproductively old mice exhibit more failed ovulation events. (A) Representative H&E-stained ovarian tissue sections depicting two phenotypes indicative of failed ovulation events including (I) trapped oocytes within CLs and (II) expanded unruptured antral follicles. Scale bars are 100 μm. (B) Graph showing the percent of failed ovulations per CL number for reproductively young and old mice (N = 10 ovaries per age group). A t-test was performed and P = 0.025. Data are represented as mean ± SEM. The number of failed ovulations per ovary for all (C) reproductively young and (D) reproductively old mice. (E) Representative H&E-stained ovarian tissue section depicting a hemorrhagic CL. The scale bar is 50 μm. (F) Graph showing the percent of hemorrhagic CLs for both age cohorts (N = 10 ovaries per age group). A t-test was performed and there was no significant difference (P > 0.05). Data are represented as mean ± SEM. The percent of hemorrhagic CLs for all (G) reproductively young and (H) reproductively old mice.