Research Paper Volume 12, Issue 10 pp 9714—9725

Qigesan inhibits esophageal cancer cell invasion and migration by inhibiting Gas6/Axl-induced epithelial-mesenchymal transition

Figure 2. QGS regulates the localization and expression of Gas6/Axl. Cells were stimulated with QGS (0, 100, or 200 μg/mL) for 24 h. (A, B) Immunofluorescence images of Gas6, Axl, and Gas6/Axl channels in ECA109 and TE13 cells. Scale bars indicate 10 μm. (C, D) Western immunoblots of Gas6 and Axl. Bar graphs show significant dose-dependent inhibition of relative Gas6 and Axl protein density (normalized to β-act) after QSG stimulation compared to the unstimulated control group. Results are from three independent experiments. *p<0.05, **p<0.01, Gas6 relative protein density compared to control; &p<0.05, &&p<0.01, Axl relative protein density compared to control.