Figure 4. HPLC analysis of metabolites in CTL and macroH2A1 KD UM 92.1 cells. (A) Heatmap representing the levels of major classes of metabolites detected by HPLC. (B) Acetyl-co-A levels; (C) NADP+ levels; (D) NADPH levels; (E) NADP+/NADPH levels; (F) NAD+/NADH levels. Results are presented as the mean ± SEM of four independent experiments. (*p < 0.01; *** p < 0.001 vs control).