Research Paper Volume 12, Issue 10 pp 9761—9780

Physiological and metabolic features of mice with CRISPR/Cas9-mediated loss-of-function in growth hormone-releasing hormone

Figure 2. Suppression of growth hormone signaling. Expression of growth hormone gene in pituitary gland in female (A) and male (B) WT and GHRH-/- mice. Expression of prolactin gene in pituitary gland in female (C) and male (D) WT and GHRH-/- mice. Expression of IGF-1 gene in liver in female (E) and male (F) WT and GHRH-/- mice. Expression levels are shown as relative expression compared to WT mice. For all biological groups n=5. Each bar represents means. Statistical analysis was performed by unpaired Student’s t-test with Welch’s correction; ns= not significant, **p<0.01, ***p<0.001, ****p<0.0001.