Research Paper Volume 12, Issue 10 pp 9761—9780

Physiological and metabolic features of mice with CRISPR/Cas9-mediated loss-of-function in growth hormone-releasing hormone

Figure 5. Respiratory exchange ratio (VCO2/VO2). RER values were calculated by dividing VCO2 with VO2. 6 days of female (A) and male (B) WT and GHRH-/- mice RER values are shown. Hourly averaged RER values on day of female (C) and male (D) WT and GHRH-/- mice. Overall averaged RER values are shown as light (E, F) and dark cycles (G, H) for female (E, G) and male (F, H) WT and GHRH-/- mice. Female WT n=12, GHRH-/- n=12, male WT n=12, GHRH-/- n=11. Each bar represents mean ± SEM. Statistical analysis was performed by unpaired Student’s t-test with Welch’s correction; ns= not significant, a; *p<0.05, b; **p<0.01, c; ***p<0.001, d; ****p<0.0001.