Figure 9. ANCOVA shows that GH-deficiency reduces metabolic rate. Energy expenditure values for 6 days for female (A) and male (B) GHRH-/- and WT mice. 6 days of energy expenditure data were averaged into a single day for female (C) and male (D) mice. Analysis of energy expenditure with body weight as a co-variant for female (E, G) and male (F, H) GHRH-/- and WT mice in light cycles (E, F) and dark cycles. (G, H) Female WT n=12, GHRH-/- n=12, male WT n=12, GHRH-/- n=11. Each bar represents mean ± SEM. The WT and GHRH-/- groups were statistically analyzed with ANCOVA method, which was used to calculate p values, shown on panels E-H.