Research Paper Volume 12, Issue 10 pp 9781—9792

LncRNA DSCAM-AS1 promotes colorectal cancer progression by acting as a molecular sponge of miR-384 to modulate AKT3 expression

Figure 4. DSCAM-AS1 acted as a sponge for miR-384. (A) The predicted binding sites of miR-384 on the sequence of DSCAM-AS1(WT-DSCAM-AS1). The target sequences of the DSCAM-AS1 were mutated (MT-DSCAM-AS1). (B) Luciferase activity was examined in LOVO and HT29 cells co-transfected with miR-384 mimics or miR-NC, and luciferase reporter vector containing WT-DSCAM-AS1 or MT-DSCAM-AS1. WT: wild-type; MT: mutant-type. (C) The interaction between miR-384 and DSCAM-AS1 was determined in LOVO and HT29 cells with RIP assay. (D) The expression of miR-384 in LOVO and HT29 cells cells transfected with sh-NC or sh-DSCAM-AS1 was determined by qRT-PCR. (E) The expression of DSCAM-AS1 in LOVO and HT29 cells transfected with miR-NC or miR-384 mimic was determined by qRT-PCR. (F) qRT-PCR shows the miR-384 expression level in 56 pairs CRC tissues and non-tumor tissues. (G) Pearson's correlation analysis between miR-384 expression and DSCAM-AS1 expression in 56 CRC tissues. P< 0.05, **P< 0.01.