Research Paper Volume 12, Issue 10 pp 9793—9806

Circular RNA hsa_circ_0003141 promotes tumorigenesis of hepatocellular carcinoma via a miR-1827/UBAP2 axis

Figure 5. Hsa_circ_0003141 functions as a ceRNA of miR-1827 in HCC. (A, B) The 3'-UTR of hsa_circ_0003141 harbors miR-1827 cognate sites. (C) The level of miR-1827 in Huh-7 cells transfected with miR-1827 mimics or miR-1827 inhibitor analyzed by qRT-PCR. (D) Relative luciferase activity of reporter plasmids carrying WT- or MT-hsa_circ_0003141 3'-UTR in Huh-7 cells transfected with miR-1827, and analyzed using dual luciferase reporter assay. (E) Cellular localization of hsa_circ_0003141 and miR-1827 in Huh-7 cells was analyzed using FISH assay. **P < 0.01, compared with the vector-control group.