Figure 10. The overexpression of LINC00402, LINC00461, and SFTA1P suppresses tumor formation in nude mice. Nude mice were injected with cell suspension carrying HT-29 cells transfected with NC, PHLPP2, PHLPP + pcDNA-LINC00402, PHLPP + pcDNA-LINC00461 or PHLPP + pcDNA-SFTA1P. (A) The representative images of formed tumors among the nude mice. (B) Tumor growth curve of nude mice. (C) Tumor weight of nude mice. (D) RT-qPCR was carried out to detect the expressions of PHLPP2 mRNA, miR-141 and miR-424 in nude mice (E, F) Protein levels of PHLPP2 proteins, Akt3 and p27 in nude mice normalized to GAPDH detected by Western blot analysis. * p < 0.05 vs. the NC group; # p < 0.05 vs. the pcDNA-LINC00402, pcDNA-LINC00461 and pcDNA-SFTA1P groups. Measurement data in this Figure were expressed as the mean ± standard deviation, and comparisons among multiple groups conducted using One-Way ANOVA (n = 5); the experiment was repeated three times independently.