Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Figure 10. The overexpression of LINC00402, LINC00461, and SFTA1P suppresses tumor formation in nude mice. Nude mice were injected with cell suspension carrying HT-29 cells transfected with NC, PHLPP2, PHLPP + pcDNA-LINC00402, PHLPP + pcDNA-LINC00461 or PHLPP + pcDNA-SFTA1P. (A) The representative images of formed tumors among the nude mice. (B) Tumor growth curve of nude mice. (C) Tumor weight of nude mice. (D) RT-qPCR was carried out to detect the expressions of PHLPP2 mRNA, miR-141 and miR-424 in nude mice (E, F) Protein levels of PHLPP2 proteins, Akt3 and p27 in nude mice normalized to GAPDH detected by Western blot analysis. * p < 0.05 vs. the NC group; # p < 0.05 vs. the pcDNA-LINC00402, pcDNA-LINC00461 and pcDNA-SFTA1P groups. Measurement data in this Figure were expressed as the mean ± standard deviation, and comparisons among multiple groups conducted using One-Way ANOVA (n = 5); the experiment was repeated three times independently.