Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

class="figure-viewer-img"

Figure 3. The expression of PHLPP2 decreases while that of miR-141 and miR-424 increases in colon cancer tissues. (A) Measurement of the expression of PHLPP2 mRNA in colon cancer tissues and adjacent tissues by RT-qPCR. (B) Determination of the expression of miR-141 in colon cancer tissues and adjacent tissues by RT-qPCR. (C) Determination of the expression of miR-424 in colon cancer tissues and adjacent tissues by RT-qPCR. (D) Pearson’s Correlation analysis of PHLPP2 and miR-141 in colon cancer. (E) Pearson’s Correlation analysis of PHLPP2 and miR-424 in colon cancer. (F) Survival time of patients with high and low expression of PHLPP2 analyzed by Kaplan-Meier survival analysis. (G) Immunohistochemistry and ISH were applied to identify the expression of PHLPP2, miR-141, and miR-424 in colon cancer tissues and adjacent tissues (200 ×). * p < 0.05 vs. the adjacent tissues; Measurement data in this Figure are expressed as the mean ± standard deviation, with the comparisons between the two groups conducted using paired t-test, n = 135; the experiment was repeated three times independently.