Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Figure 3. The expression of PHLPP2 decreases while that of miR-141 and miR-424 increases in colon cancer tissues. (A) Measurement of the expression of PHLPP2 mRNA in colon cancer tissues and adjacent tissues by RT-qPCR. (B) Determination of the expression of miR-141 in colon cancer tissues and adjacent tissues by RT-qPCR. (C) Determination of the expression of miR-424 in colon cancer tissues and adjacent tissues by RT-qPCR. (D) Pearson’s Correlation analysis of PHLPP2 and miR-141 in colon cancer. (E) Pearson’s Correlation analysis of PHLPP2 and miR-424 in colon cancer. (F) Survival time of patients with high and low expression of PHLPP2 analyzed by Kaplan-Meier survival analysis. (G) Immunohistochemistry and ISH were applied to identify the expression of PHLPP2, miR-141, and miR-424 in colon cancer tissues and adjacent tissues (200 ×). * p < 0.05 vs. the adjacent tissues; Measurement data in this Figure are expressed as the mean ± standard deviation, with the comparisons between the two groups conducted using paired t-test, n = 135; the experiment was repeated three times independently.