Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Figure 4. Overexpressed PHLPP2 inhibits the proliferation, migration, invasion of HT-29 cells. HT-29 cells were transfected with NC-pcDNA, PHLPP2, NC-inh, miR-141 inhibitor, miR-424 inhibitor, PHLPP2 + miR-141 or PHLPP2 + miR-424. (A, E) EdU labeling detected HT-29 cell proliferation (200 ×). (B, F) Flow cytometry analyzed apoptotic HT-29 cells. (C, G) Transwell assay detected HT-29 cell migration (100 ×). (D, H) Scratch test detected invasive HT-29 cells 48 h after treatment. (I) Transfection efficiency detected by RT-qPCR. * p < 0.05 vs. the NC-pcDNA group; # p < 0.05 vs. the NC-inh group; &, p < 0.05 vs. the PHLPP2 group; Measurement data in this Figure are expressed as the mean ± standard deviation, while comparisons among multiple groups were conducted using One-Way ANOVA; the experiment was repeated three times independently.