Figure 4. Overexpressed PHLPP2 inhibits the proliferation, migration, invasion of HT-29 cells. HT-29 cells were transfected with NC-pcDNA, PHLPP2, NC-inh, miR-141 inhibitor, miR-424 inhibitor, PHLPP2 + miR-141 or PHLPP2 + miR-424. (A, E) EdU labeling detected HT-29 cell proliferation (200 ×). (B, F) Flow cytometry analyzed apoptotic HT-29 cells. (C, G) Transwell assay detected HT-29 cell migration (100 ×). (D, H) Scratch test detected invasive HT-29 cells 48 h after treatment. (I) Transfection efficiency detected by RT-qPCR. * p < 0.05 vs. the NC-pcDNA group; # p < 0.05 vs. the NC-inh group; &, p < 0.05 vs. the PHLPP2 group; Measurement data in this Figure are expressed as the mean ± standard deviation, while comparisons among multiple groups were conducted using One-Way ANOVA; the experiment was repeated three times independently.