Figure 5. Overexpressed PHLPP2 suppresses the EMT process and tumorigenicity of HT-29 cells. HT-29 cells were transfected with NC-pcDNA, PHLPP2, NC-inh, miR-141 inhibitor, miR-424 inhibitor, PHLPP2 + miR-141 or PHLPP2 + miR-424. (A) The expressions of EMT-related factors (E-cadherin, N-cadherin, and Vimentin) detected by immunofluorescence assay in HT-29 cells (200 ×). (B) Western blot analysis of EMT-related factors (E-cadherin, N-cadherin and Vimentin), apoptosis factors (Bax and cleaved Caspase-3) and Akt3-p27 pathway-related proteins (Akt1, Akt3, and p27) in HT-29 cells normalized to GAPDH. (C) The representative images of formed tumors in nude mice. (D) Tumor growth of nude mice. (E) Tumor weight of nude mice. * p < 0.05 vs. the NC-pcDNA group; # p < 0.05 vs. the NC-inh group; & p < 0.05 vs. the PHLPP2 group; Measurement data in this Figure expressed as the mean ± standard deviation, with comparisons between multiple groups conducted using One-Way ANOVA (n = 5); the experiment was repeated three times independently.