Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Figure 5. Overexpressed PHLPP2 suppresses the EMT process and tumorigenicity of HT-29 cells. HT-29 cells were transfected with NC-pcDNA, PHLPP2, NC-inh, miR-141 inhibitor, miR-424 inhibitor, PHLPP2 + miR-141 or PHLPP2 + miR-424. (A) The expressions of EMT-related factors (E-cadherin, N-cadherin, and Vimentin) detected by immunofluorescence assay in HT-29 cells (200 ×). (B) Western blot analysis of EMT-related factors (E-cadherin, N-cadherin and Vimentin), apoptosis factors (Bax and cleaved Caspase-3) and Akt3-p27 pathway-related proteins (Akt1, Akt3, and p27) in HT-29 cells normalized to GAPDH. (C) The representative images of formed tumors in nude mice. (D) Tumor growth of nude mice. (E) Tumor weight of nude mice. * p < 0.05 vs. the NC-pcDNA group; # p < 0.05 vs. the NC-inh group; & p < 0.05 vs. the PHLPP2 group; Measurement data in this Figure expressed as the mean ± standard deviation, with comparisons between multiple groups conducted using One-Way ANOVA (n = 5); the experiment was repeated three times independently.